| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Bacillus subtilis | TGCGCACCTTGCAAGACT | ACCGGAAGACCTTTACCCG | 60.20 | 59.02 | 186 | 85 |
100.00%
|
100.00%
|
| Primer 42 | Bacillus subtilis | ACAGGCTGCTGATTTTGTGC | GCCCTTTTCCCCAATCCCA | 59.68 | 59.92 | 197 | 85 |
100.00%
|
100.00%
|
| Primer 43 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | TGTTCCCGACTCCGTTGAC | 59.28 | 59.64 | 272 | 85 |
100.00%
|
100.00%
|
| Primer 44 | Bacillus subtilis | TCCGTTTCGCCAACTGGAA | AATGCAGACCGAGCAGCA | 59.85 | 59.65 | 171 | 84 |
100.00%
|
100.00%
|
| Primer 45 | Bacillus subtilis | CGCCAACTGGAACGGAGAA | AATGCAGACCGAGCAGCA | 60.30 | 59.65 | 164 | 84 |
100.00%
|
100.00%
|
| Primer 46 | Bacillus subtilis | TAGAGGATGGCGAGGTGCT | TTGACGCTTTCCCGTGCA | 60.08 | 60.20 | 289 | 84 |
100.00%
|
100.00%
|
| Primer 47 | Bacillus subtilis | TGCTAGAGGATGGCGAGGT | TTGACGCTTTCCCGTGCA | 60.08 | 60.20 | 292 | 84 |
100.00%
|
100.00%
|
| Primer 48 | Bacillus subtilis | ATGCTCCAAGCGCTGGTA | CAAAAGCGGTTGCCAGCA | 59.01 | 59.58 | 199 | 83 |
100.00%
|
100.00%
|
| Primer 49 | Bacillus subtilis | GCACAGCATGCGAAACAGT | ATGGCGTTCCCGTGATGA | 59.72 | 59.01 | 221 | 82 |
100.00%
|
50.00%
|
| Primer 50 | Bacillus subtilis | AAAACGTGCAGCACCTGTG | TCAGCACGGCTTCAAGCA | 59.57 | 59.89 | 178 | 82 |
100.00%
|
100.00%
|